Able to identify over 6,000 different types of bacteria in about 20 seconds.
In order to prescribe the best antiobiotic to fight off infection, we must know the type of bacteria causing the infection. Traditionally, the process to identify the type of bacateria could take 12-48 hours.
With the MALDI-TOF Technology, bacterial identification is reduced to 20 seconds! Armed sooner with this information, physicians can prescribe appropriate antibiotics much faster.
Every bacteria has a unique protein “fingerprint.” Thus, if we can determine the fingerprint of a bacteria, we know its identify.
The MALDI-TOF is a mass spectrometer that measures the size and charge of the bacterial “fingerprint” in order to identify the specific bacteria type.
Step 1 - Plating and Growing the Bacteria
Specimens from a suspected infected site are sent by a physician to EIRMC’s microbiology department. The microbiologis’s job is to grow bacteria from the specimen to identify its type. Specimens are plated onto specialized agar plates, each containing different nutrients to support growth of different types of bacteria. The plates are incubated for 18-24 hours.
Step 2 - Identifying the Bacteria:
Once colonies have grown, Microbiologist places the unidentified bacteria on a metal plate with targets etched into it. Once dried, a solution containing crystallized molecules (called “matrix”) is added to the bacteria on the targets. As matrix dries, it binds specifically to the proteins in the bacteria.
The plate is then placed in the loading platform of the MALDI-TOF, and a laser is lined up on the targets.
When the laser hits the target, the resulting energy breaks apart the bacteria and releases its protein. The laser’s energy also causes the matrix, which is bound to the protein, to transfer a positive charge to the protein.
The positive charge allows the protein to “fly” to the top of the vacuum chamber.
The “time of flight” for the set of proteins to travel to the top of the vacuum chamber is measured.
This measurement tells the microbiologist which proteins are present in the bacteria. With this unique protein “fingerprint,” a positive ID of the bacterial type can be made.
Step 3 - Testing to Fight the Bacteria
Now, we confirm which antibiotics are best to kill the patient’s infection. The bacteria is again placed in wells, which contain various antibiotics at different concentrations, instead of biochemicals. The Microbiologist reviews the wells after 18 hours; based on bacterial growth- or lack of- we can determine if the bacteria is sensitive to an antibiotic (a good thing) or resistant to it (not a good thing).